Journal: Journal of Ginseng Research

Article Title: Inhibitory effects of ginsenosides on basic fibroblast growth factor-induced melanocyte proliferation

doi: 10.1016/j.jgr.2016.05.001

Figure Lengend Snippet: Effects of ginsenosides Rh1, CK, Rh3 and F1 on bFGF-induced ERK activation in melanocytes. Melan-a melanocytes were cultured in 60-mm dishes (1x10 6 cells/dish). After 24 h, cells were pretreated with ginsenoside (10 μM) (A) CK, (B) Rh1, (C) Rh3, (D) F1 and (E) Rh3 and F1 for 24 h. Cells were treated with bFGF (10 ng/mL) at the indicated times for Western blot analysis of phospho-ERK (p-ERK) and total-ERK (t-ERK). Band intensity was quantified and normalized relative to ERK using ImageJ (1.47) software (NIH, USA).

Article Snippet: Ginsenoside compounds were purchased from Chengdu Biopurify Phytochemicals, Ltd (Chengdu, Sichuan, China).

Techniques: Activation Assay, Cell Culture, Western Blot, Software

Journal: Journal of Ginseng Research

Article Title: Inhibitory effects of ginsenosides on basic fibroblast growth factor-induced melanocyte proliferation

doi: 10.1016/j.jgr.2016.05.001

Figure Lengend Snippet: Effects of ginsenosides Rh3 and F1 on bFGF-induced MITF expression in melanocytes. Melan-a melanocytes were cultured in 60 mm dishes (1x10 6 cells/dish). After 24 h, cells were pretreated with ginsenoside (10 μM) (A) Rh3, (B) F1 and (C) Rh3 and F1 for 24 h. Cells were treated with bFGF (10 ng/mL) at the indicated times for Western blot analysis of MITF. Band intensity was quantified and normalized relative to β-actin using ImageJ (1.47) software (NIH, USA).

Article Snippet: Ginsenoside compounds were purchased from Chengdu Biopurify Phytochemicals, Ltd (Chengdu, Sichuan, China).

Techniques: Expressing, Cell Culture, Western Blot, Software

Journal: International Journal of Molecular Sciences

Article Title: Chebulinic Acid Suppresses Adipogenesis in 3T3-L1 Preadipocytes by Inhibiting PPP1CB Activity

doi: 10.3390/ijms23020865

Figure Lengend Snippet: Identification of chebulinic acid as an anti-obesity drug candidate by screening of 1033 natural products for PPP1CB inhibitors. A total of 1033 natural products were transferred to black/black bottom 96-well plates and enzymatic screening was performed with PPP1CB. Inhibitory activity of each compound was evaluated using 6,8-difluoro-4-methylumbelliferyl phosphate (DiFMUP)-based assays, and the inhibitory effects of the 1033 natural products on PPP1CB were calculated using control-based normalization. The top 45 hits were selected. Among the 45 hits, nine compounds that have not reported anti-adipogenic effects were sorted based on literature search. These nine selected compounds were evaluated using oil red O staining to determine whether they suppress adipogenesis in 3T3-L1 cells. Finally, chebulinic acid was selected as the most potent compound for obesity treatment.

Article Snippet: To test the inhibitory effects of the 1033 compounds (Chengdu Biopurify, Chengdu, China) on PPP1CB, each compound was dissolved in DMSO (200 μM) and transferred to polypropylene (PP) 96-well plates.

Techniques: Activity Assay, Control, Staining

Journal: Chinese Medicine

Article Title: A multivariate analysis on the comparison of raw notoginseng ( Sanqi ) and its granule products by thin-layer chromatography and ultra-performance liquid chromatography

doi: 10.1186/s13020-015-0040-2

Figure Lengend Snippet: Regression data, detection/quantification limits and precision data for the five compounds determined by UPLC-PDA

Article Snippet: The five marker compounds, notoginseng NR1 and ginsenosides Rg1, Rb1, Rd and Rg2, were purchased from Chengdu Biopurify Phytochemicals Ltd. (Chengdu, China; purity >98 %).

Techniques:

Journal: Chinese Medicine

Article Title: A multivariate analysis on the comparison of raw notoginseng ( Sanqi ) and its granule products by thin-layer chromatography and ultra-performance liquid chromatography

doi: 10.1186/s13020-015-0040-2

Figure Lengend Snippet: Contents (mg/g, mean ± SD, n = 3) of the five compounds in Sanqi samples analysed by TLC and UPLC-PDA

Article Snippet: The five marker compounds, notoginseng NR1 and ginsenosides Rg1, Rb1, Rd and Rg2, were purchased from Chengdu Biopurify Phytochemicals Ltd. (Chengdu, China; purity >98 %).

Techniques:

Journal: Chinese Medicine

Article Title: A multivariate analysis on the comparison of raw notoginseng ( Sanqi ) and its granule products by thin-layer chromatography and ultra-performance liquid chromatography

doi: 10.1186/s13020-015-0040-2

Figure Lengend Snippet: PCA biplot (loading and score plot) of Sanqi raw herb and granule extracts analysed by ( a ) TLC and ( b ) UPLC. a From the TLC biplot, R1 to R4 demonstrated a higher amount of the compounds (Cluster 1), especially R4 (highest amount of Rd and Rg2). R5 and R6 were closely distributed with the granules (Cluster 2). b For UPLC, Group 1 containing the raw herbs, G7 and G12 which possessed higher amounts of the five compounds, whilst Group 2 contained the rest of the granules

Article Snippet: The five marker compounds, notoginseng NR1 and ginsenosides Rg1, Rb1, Rd and Rg2, were purchased from Chengdu Biopurify Phytochemicals Ltd. (Chengdu, China; purity >98 %).

Techniques:

Journal: Chinese Medicine

Article Title: A multivariate analysis on the comparison of raw notoginseng ( Sanqi ) and its granule products by thin-layer chromatography and ultra-performance liquid chromatography

doi: 10.1186/s13020-015-0040-2

Figure Lengend Snippet: Pearson correlation between the contents of the compounds analysed by UPLC and the anti-oxidant capacity of the extracts

Article Snippet: The five marker compounds, notoginseng NR1 and ginsenosides Rg1, Rb1, Rd and Rg2, were purchased from Chengdu Biopurify Phytochemicals Ltd. (Chengdu, China; purity >98 %).

Techniques:

Journal: Veterinary World

Article Title: Treatment efficacy of Thunbergia laurifolia , Curcuma longa , Garcinia mangostana , and Andrographis paniculata extracts in Staphylococcus aureus -induced rabbit dermatitis model

doi: 10.14202/vetworld.2022.188-197

Figure Lengend Snippet: Phytochemical screening of ethanol crude extracts.

Article Snippet: The phytochemical compounds found in the Thai herb Thunbergia laurifolia Lindl., belonging to the Acanthaceae family, include sterols (beta-sitosterol, stigmasterol, and alpha-spinasterol), phenolic compounds (gallic acid, apigenin, protocatechuic, and caffeic acid), carotenoid compounds (lutein), unclassified steroids, and glycoside [ ].

Techniques: